25/11/2010 : Séminaire de microscopie AFM : Living Cerebral Endothelial Cells Investigated by AFM
by Attila Gergely Biophysics Institute Biological Research Center Hungarian Academy of Sciences 6726 Szeged , Hungary
After the invention of the atomic force microscope (AFM), the instrument evolved as a high resolution noninvasive microscope. Besides of high resolution imaging. In the native environment of biological samples, the AFM is a powerful tool to determine the mechanical properties of the studied object, such as elasticity of living cells. Cerebral endothelial cells interconnected by tight and adherens junctions constitute the structural basis of the blood-brain barrier. Understanding the reaction of living cells in response to different extracellular stimuli, such as hyperosmotic stress, extracellular calcium depletion, is of primordial importance. Morphological and elastic parameters of living cerebral endothelial cells were investigated under different circumstances by AFM.